Papers

#Equal contribution, Co-corresponding

Preprints

#Pedram, K., #Shon, D. J., #Tender, G. S., Mantuano, N. R., Northey, J. J., Metcalf, K. J., Wisnovsky, S. P., Riley, N. M., Forcina, G. C., Malaker, S. A., Kuo, A., George, B. M., Miller, C. L., Casey, K. M., Vilches-Moure, J. G., Weaver, V. M., Laübli, H. & Bertozzi, C. R.
Design of a mucin-selective protease for targeted degradation of cancer-associated mucins. bioRxiv (2022). doi:10.1101/2022.05.20.492748


Publications

Almahayni, K., Spiekermann, M., Fiore, A., Yu, G., Pedram, K. & Möckl, L. Small molecule inhibitors of mammalian glycosylation. Matrix Biology Plus 16, 100108 (2022).

Pedram, K., Laqtom, N. N., Shon, D. J., Di Spiezio, A., Riley, N. M., Saftig, P., Abu-Remaileh, M. & Bertozzi, C. R. Lysosomal cathepsin D mediates endogenous mucin glycodomain catabolism in mammals. PNAS 119, e2117105119 (2022).

#Malaker, S. A., #Riley, N. M., Shon, D. J., Pedram, K., Krishnan, V., Dorigo, O. & Bertozzi, C. R. Revealing the human mucinome. Nat Commun 13, 3542 (2022).

Lu, C.-H., Pedram, K., Tsai, C.-T., Jones, T., Li, X., Nakamoto, M. L., Bertozzi, C. R. & Cui, B. Membrane curvature regulates the spatial distribution of bulky glycoproteins. Nat Commun 13, 3093 (2022).

Flynn, R. A., Pedram, K., Malaker, S. A., Batista, P. J., Smith, B. A. H., Johnson, A. G., George, B. M., Majzoub, K., Villalta, P. W., Carette, J. E. & Bertozzi, C. R. Small RNAs are modified with N-glycans and displayed on the surface of living cells. Cell 184, 3109-3124.e22 (2021).

Wisnovsky, S., Möckl, L., Malaker, S. A., Pedram, K., Hess, G. T., Riley, N. M., Gray, M. A., Smith, B. A. H., Bassik, M. C., Moerner, W. E. & Bertozzi, C. R. Genome-wide CRISPR screens reveal a specific ligand for the glycan-binding immune checkpoint receptor Siglec-7. PNAS 118, e2015024118 (2021).

Imbert, P. R. C., Saric, A., Pedram, K., Bertozzi, C. R., Grinstein, S. & Freeman, S. A. An Acquired and Endogenous Glycocalyx Forms a Bidirectional “Don’t Eat” and “Don’t Eat Me” Barrier to Phagocytosis. Current Biology 31, 77-89.e5 (2021).

Shon, D. J., Malaker, S., Pedram, K., Yang, E., Krishnan, V., Dorigo, O. & Bertozzi, C. An Enzymatic Toolkit for Selective Proteolysis, Detection, and Visualization of Mucin-Domain Glycoproteins. PNAS 117, 21299-307 (2020).

Banik, S. M., Pedram., K., Wisnovsky, S., Riley, N. M., and Bertozzi, C. R. Lysosome-targeting chimaeras for degradation of extracellular proteins. Nature 584, 291–297 (2020).

Schumann, B., Malaker, S. A., Wisnovsky, S. P., Debets, M. F., Agbay, A. J., Fernandez, D., Wagner, L. J. S., Lin, L., Li, Z., Choi, J., Fox, D. M., Peh, J., Gray, M. A., Pedram, K., Kohler, J. J., Mrksich, M. & Bertozzi, C. R. Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells. Molecular Cell 78, 824-834.e15 (2020).

#Shurer, C. R., #Kuo, J. C.-H., #Roberts, L. M., Gandhi, J. G., Colville, M. J., Enoki, T. A., Pan, H., Su, J., Noble, J. M., Hollander, M. J., O’Donnell, J. P., Yin, R., Pedram, K., Möckl, L., Kourkoutis, L. F., Moerner, W. E., Bertozzi, C. R., Feigenson, G. W., Reesink, H. L. & Paszek, M. J. Physical Principles of Membrane Shape Regulation by the Glycocalyx. Cell 177, 1757–1770 (2019).

#Möckl, L., #Pedram, K., Roy, A. R., Krishnan, V., Gustavsson, A.-K., Dorigo, O., Bertozzi, C. R., and Moerner, W. E. Quantitative super-resolution microscopy of the mammalian glycocalyx. Developmental Cell 50, 57-72 (2019).
Featured on the cover of volume 50, issue 1

#Malaker, S. A., #Pedram, K., Ferracane, M. J., Bensing, B. A., Krishnan, V., Pett, C., Yu, J., Woods, E. C., Kramer, J. R., Westerlind, U., Dorigo, O. & Bertozzi, C. R. The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. PNAS 116, 7278–87 (2019).
Featured in Stanford ChEM-H News

Woods, E. C., Kai, F., Barnes, J. M., Pedram, K., Pickup, M. W., Hollander, M. J., Weaver, V. M. & Bertozzi, C. R. A bulky glycocalyx fosters metastasis formation by promoting G1 cell cycle progression. eLife 6, e25752 (2017).

Udeshi, N. D., Pedram, K., Svinkina, T., Fereshetian, S., Myers, S. A., Aygun, O., Krug, K., Clauser, K., Ryan, D., Ast, T., Mootha, V. K., Ting, A. Y. & Carr, S. A. Antibodies to biotin enable large-scale detection of biotinylation sites on proteins. Nature Methods 14, 1167-1170 (2017).

Hung, V., Udeshi, N. D., Lam, S. S., Loh, K. H., Cox, K. J., Pedram, K., Carr, S. A., & Ting, A. Y. Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2. Nature Protocols 11, 456-475 (2016).

Hauptman, J. S., Pedram, K., Sison, C. A., Sankar, R., Salamon, N., Vinters, H. V. & Mathern, G. W. Pediatric Epilepsy Surgery: Long-term 5-Year Seizure Remission and Medication use. Neurosurgery 71, 985–993 (2012).